Liquid-Chromatography2019-09-10T14:11:53+02:00

Liquid Chromatography

Separate even the most complex samples with confidence. With an extensive portfolio of innovative Thermo Scientific HPLC and UHPLC systems, you can choose the best system for your application. Learn which system is right for you, and customize it to meet your separation needs.

Vanquish UHPLC Systems

Solid-phase extraction (SPE) is a technique for liquid-liquid extraction that is used to remove certain compounds from a mixture of impurities based on their physical and chemical properties. Analytical laboratories use SPE to concentrate and purify samples for analysis. SPE can be used to isolate analytes of interest from a wide variety of matrices, including urine, blood, beverage, and water samples. SPE requirements vary depending on the amount of sample required. SPE systems can be separated into large or small volume techniques. Large volume techniques generally use 100 mL or more of liquid sample.

Vanquish™ Horizon UHPLC System

For your most complex and difficult samples, or for applications where you are aiming for the shortest cycle times and highest throughput. Vanquish Horizon UHPLC system allows for smaller particles and column dimensions to be run with greater confidence for the most demanding LC and LC-MS workflows.

For assays that require more flexibility in solvents to accommodate all applications. Vanquish Flex UHPLC systems excel from method development to routine analyses. With binary and quaternary pump options, the Vanquish Flex UHPLC systems offer outstanding performance for targeted LC and LC-MS workflows.

Vanquish™ Duo UHPLC Systems

Increase productivity, up-time, and sample knowledge for a faster return on investment. Vanquish Duo UHPLC systems expands the benefits of the Vanquish platform through two flow paths to support three workflows (dual, tandem and inverse gradient) within one integrated UHPLC system. With the addition of the second flow path, improve your productivity by saving time, cost per sample without added bench space, and without compromising performance, robustness or ease-of-use.

Vanquish™ Horizon UHPLC System

This integrated, fully biocompatible system provides you with unrivaled performance and throughput for applications requiring high-end UHPLC.

  • Unsurpassed retention time and peak area precision
  • Highest detector sensitivity and lower baseline noise
  • Less maintenance and easy set-up with Thermo Scientific Viper Fingertight Fittings
  • Dedicated solutions for exceptional LC-MS performance
System pressure Up to 1500 bar
Retention time precision
Flow range 0 – 5 mL/min
Biocompatibility
Design Modular
SmartFlow technology for excellent flow precision
Pump High-pressure gradient proportioning
Active and passive solvent pre-heating
Multi-mode column thermostatting
Sample cooling Temperature range: 4 – 40 °C
Pump Vanquish Binary Pump H The pump with the highest backpressure at 1500 bar; ideal for any gradient performance, from shallow to ballistic
Autosampler Vanquish Split Sampler HT Excellent precision, accuracy, and short cycle times with innovative air-stream cooling and barcode reading for inventory management for all workflows up to 1500 bar
Extended sample capacity Vanquish Charger (optional) An intelligent, robotic rack and plate feeder that automatically loads samples in the autosampler and extends the sample capacity of all Vanquish samplers to up to 8832 samples (23 wellplates or 12 vial racks) with innovative air-stream cooling and barcode reading for inventory management.
Column compartment Vanquish Column Compartment Provides two column thermostatting modes (still and forced air), active and passive solvent pre-conditioning and post-column cooling for optimized control of your separations and advanced method transfer.
Virtually zero dead volume system connections Viper UHPLC Fittings Kits Order all the UHPLC fittings and capillaries you need for your system or application in a single kit
  • Biocompatible with a state-of-the-art binary high-pressure pump and state-of-the-art binary solvent blending
  • Ideal for extended throughput that can be seamlessly coupled to a mass spectrometer (MS).
  • A biocompatible UHPLC system with state-of-the-art low-pressure quaternary solvent blending.
  • Ideal for pharmaceutical and biopharmaceutical applications.
Vanquish™ Flex Binary UHPLC System Vanquish™ Flex Quaternary UHPLC System
High pressure binary solvent blending for fast and reliable UHPLC Low pressure quaternary solvent blending for flexible method development
Solvent blending Up to 1000 bar Up to 1000 bar
Retention time precision 0-8 mL/min 0-8 mL/min
Flow range
Biocompatibility
Design Modular Modular
SmartInject technology for unsurpassed retention time precision
SmartFlow technology for excellent flow precision
Pump High-pressure gradient proportioning High-pressure gradient proportioning
Active and passive pre-heating
Multi-mode column thermostatting

Vanquish™ Duo system for Dual LC

Double your throughput or deepen your sample knowledge. Run the same method on both flow paths or two different methods simultaneously on one instrument, improving your lab productivity, sample knowledge, bench space usage and overall return on investment.

Vanquish™ Duo system for Tandem LC or LC-MS

Ready to improve your lab throughput and get the most out of your detector or mass spectrometer? The Vanquish™ Duo system for Tandem LC or LC-MS system accomplishes this by running your analysis on two pumps and two columns all on one system. Deliver the analytical gradient on the first pump; while the second pump reconditions the column—preparing for the next injection.

Vanquish™ Duo system for Inverse Gradient

Improve your ability to detect and quantify all compounds in your sample with universal Charged Aerosol Detection using an Inverse Gradient. Advance your quantification of unknowns, without internal standards, and see what you’ve been missing.

Overview of the Vanquish™ Duo Systems

Vanquish™ Duo system for Dual LC Vanquish™ Duo system for Tandem LC or LC-MS Vanquish™ Duo system for Inverse Gradient
Improved return on investment Double throughput or increase sample knowledge Increased detector utilization Reliable quantification with CAD
Saves bench space
Doubled sample throughput - -
Run complementary assays - -
Increased throughput for LC-MS -
Reliably quantify unknowns -
Dedicated workflow kits Vanquish Duo for Dual LC Workflow Kit Vanquish Duo for Tandem LC or LC-MS Workflow Kit Vanquish Duo for Inverse Gradient Workflow Kit
Pump One: Vanquish Dual Pump F Or A combination of two individual Vanquish pumps: Vanquish Binary Pump H Vanquish Binary Pump F Vanquish Quaternary Pump F One: Vanquish Dual Pump F Or A combination of two individual Vanquish pumps: Vanquish Binary Pump H Vanquish Binary Pump F Vanquish Quaternary Pump F One: Vanquish Dual Pump F Or A combination of two individual Vanquish pumps: Vanquish Binary Pump H Vanquish Binary Pump F Vanquish Quaternary Pump F
Autosampler Vanquish Dual Split Sampler FT For applications up to 1000 bar Or Vanquish Dual Split Sampler HT For applications up to 1500 bar Vanquish Split Sampler FT For applications up to 1000 bar Or Vanquish Split Sampler HT For applications up to 1500 bar Vanquish Split Sampler FT For applications up to 1000 bar Or Vanquish Split Sampler HT For applications up to 1500 bar
Extended Sample Capacity Vanquish Charger (optional) An intelligent, robotic rack and plate feeder that automatically loads samples in the autosampler and extends the sample capacity of all Vanquish samplers to up to 8832 samples (23 wellplates or 12 vial racks) with innovative air-stream cooling and barcode reading for inventory management. Vanquish Charger (optional) An intelligent, robotic rack and plate feeder that automatically loads samples in the autosampler and extends the sample capacity of all Vanquish samplers to up to 8832 samples (23 wellplates or 12 vial racks) with innovative air-stream cooling and barcode reading for inventory management. Vanquish Charger (optional) An intelligent, robotic rack and plate feeder that automatically loads samples in the autosampler and extends the sample capacity of all Vanquish samplers to up to 8832 samples (23 wellplates or 12 vial racks) with innovative air-stream cooling and barcode reading for inventory management.
Column compartment 1 or 2 column compartments depending on application 1 column compartment 1 column compartment
Detectors A combination of two detectors: Vanquish Charged Aerosol Detector Vanquish Diode Array Detector Vanquish Fluorescence Detector Vanquish Variable Wavelength Detector Mass Spectrometers Any detector(s): Vanquish Charged Aerosol Detector Vanquish Diode Array Detector Vanquish Fluorescence Detector Vanquish Variable Wavelength Detector Mass Spectrometers Vanquish Charged Aerosol Detector Vanquish Charged Aerosol Detector Vanquish Diode Array Detector Vanquish Fluorescence Detector Vanquish Variable Wavelength Detector Mass Spectrometers
Virtually zero dead volume system connections Viper UHPLC Fittings Kits Order all the UHPLC fittings and capillaries you need for your system or application in a single ki Viper UHPLC Fittings Kits Order all the UHPLC fittings and capillaries you need for your system or application in a single ki Viper UHPLC Fittings Kits Order all the UHPLC fittings and capillaries you need for your system or application in a single ki

UltiMate™ 3000 HPLC and UHPLC Systems

Discover why UltiMate™ 3000 LC systems are the proven workhorses of countless laboratories. These systems provide reliable chromatography and performance for a variety of applications at all scales. While being highly customizable with flexible system configurations and detector options, you can expect a fully integrated system with Chromeleon CDS software.

UltiMate™ 3000 HPLC and UHPLC Systems

The UltiMate™ 3000 intelligent LC (LCi) series delivers significant improvements in performance while simultaneously maintaining easy and reliable operation. Select from a wide range of modules to optimize your systems for numerous applications: from Rapid Separation LC (RSLC) for ultrafast high-resolution separations to sensitive nanoflow LC/MS. Whichever configuration you choose, you will get a highly integrated solution with optimum fluidic connections, single-point intelligent control through Chromeleon® software, and seamless intermodule communication.

UltiMate™ 3000 HPLC and UHPLC systems comparison

Rapid Seperation (RS) Standard (SD) Basic
System Pressure
Retention time precision
Flow range
Low dispersion and high throughput
Pump options Binary, Quaternary, Dual Binary, Quaternary, Dual Quaternary, Isocratic
Biocompatible options
Sample Cooling
Active heating column compartment
Coloum capacity
Large volume injection

Basic HPLC systems

UltiMate™ 3000 Basic Automated System

Enjoy cost effective, automated HPLC and UHPLC. Ideal for teaching or moderate throughput applications. Includes an isocratic or quaternary pump and integrated autosampler and heated column compartment

Enjoy cost effective access to HPLC and UHPLC separations. System features an isocratic pump and manual sample injection in a simple, expandable system where modularity makes anything possible.

Standard HPLC systems

Achieve stable retention times and accurate gradients in a high-throughput environment. This system is an ideal choice for fast LC and LC-MS applications, such as demanding routine analyses and high-throughput workflows in QC laboratories with a special focus on throughput, productivity, and uptime.

  • Fast gradient response
  • Excellent flow stability
  • Broad choice of high-data-rate detection technologies
  • Reliable results from every sample

Get mobile phase flexibility for your high-throughput routine or R&D separations. With a wide mixing portfolio for gradient delay volume adjustment, four-solvent gradient support, excellent flow stability and an unique choice of detection technologies, this system excels where moderately fast gradient response is acceptable to gain the advantages of improved flexibility such as in the following applications:

  • Multiple solvents
  • Extended column washes
  • Ternary or quaternary gradients
  • Method development scenarios

Increase the speed and throughput of your workflows without redeveloping existing methods. This system is ideal for routine and advanced chromatographic methods, productivity solutions, and multidimensional analyses. The dual system lets you maximize throughput, resolution, or sensitivity, and offers options for improving information yield. It is easily configured for:

  • Online-SPE
  • Orthogonal, parallel or tandem separations,
  • Inverse gradient
  • Fast application switching
  • Operation as a regular single-channel system

Rapid Separation (RS) Systems

Providing ultra-fast separations with ultra-high resolution in an exceptionally versatile HPLC/UHPLC system for the rigorous demands of large and small molecule analysis, these systems operate at pressures up to 1000 bar.

Get fast, high resolution separations with maximum flow rate flexibility. A fast gradient response and excellent flow rate stability ensure reliable results.
This is the most flexible RS system for established and novel HPLC and UHPLC workflows. The quaternary system supports fast routine applications and method development.

Increase productivity with this dual rapid separation system featuring online-SPE, parallel or tandem HPLC/UHPLC, inverse gradients and fast application switching.

Biocompatible Rapid Separation (BioRS) Systems

Get UHPLC performance for proteins, peptides, nucleic acids, and biotherapeutics. These systems are 100% corrosion free for use with high salt or extreme pH conditions. They operate with column pressures up to 1000 bar.

UltiMate 3000 BioRS

Protect your large molecules from unwanted degradation and modification. This UHPLC handles your needs from discovery to target isolation and purification.

UltiMate 3000 BioRS Dual System

Accelerate your Bio-UHPLC analyses. This dual system automates multiple UHPLC separations to combine non-compatible chemistries in one workflow.

Nano-, capillary-, and micro-flow UHPLC Systems

Are your sample volumes limited but require high sensitivity? Choose our high throughput solutions for applications such as LC-MS proteomics, metabolomics, or intact protein analysis. Achieve effortless nano-flow with our fully integrated Thermo Scientific EASY-nLC™ 1200, or leverage the flexibility of nano-, capillary-, and micro-flow rates with the customizable UltiMate™ 3000 RSLCnano system.  Both systems integrate seamlessly with Thermo Scientific mass spectrometers.

Ideal for low-flow LC-MS workflows

Do you use applications requiring high sensitivity such as proteomics, metabolomics, and intact protein analysis? The Thermo Scientific EASY-nLC™ 1200 system provides effortless nanoflow UHPLC, and the Thermo Scientific UltiMate™ 3000 RSLCnano system provides fully integrated nano-, capillary-, and micro-flow solutions.

UltiMate™ 3000 RSLCnano

A flexible, customizable system that meets all low flow applications spanning nano-, capillary-, and micro-flow needs. The UltiMate™ 3000 RSLCnano system provides excellent retention time precision for more confidence in your results.

EASY-nLC™ 1200 System

A compact, integrated solution for nano LC-MS analyses, the EASY-nLC™ 1200 offers effortless nano-flow separations and is designed for intuitive operation with excellent performance. This system can handle ultra-long columns with its high pressure capability.

Systems comparison

UltiMate™ 3000 RSLCnano EASY-nLC™ 1200 System
System Pressure
Retention time precision
Splitless nano flow
Nano flow range Recommended range: 100nL/min–1.0μL/min Recommended range: 50nL/min–1.5μL/min
Capillary flow range - Recommended range: 0.5–10μL/min
Micro flow range - Recommended range: 5–50μL/min
Design Integrated Modular
Column compartment not applicable, Built-in heating with EASY-Spray columns
Sample capacity
Sample Cooling
Zero-loss sample pick-up
Nano flow pump High pressure mixing binary syringe pump High pressure mixing binary continuous flow pump
Analytical flow and loading pump - Low pressure mixing ternary gradient pump
Direct injection
Preconcentration Vented column setup Continuous direct flow by integrated loading pump
2D salt setups -
Automated offline 2D LC* -
Offline fractionation* -
Software Xcalibur, LC Devices, & Tracefinder SII with Xcalibur & Tracefinder or Chromeleon Chromatography Data System
Integrated PC -
Audit trail -
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